Polymerase Chain Reaction:Article Title: Effects of Ice-Algal Aggregate Export on the Connectivity of Bacterial Communities in the Central Arctic Ocean
Article Snippet: .. The PCR mix for the ITS region contained 2.5 μl of 10x reaction buffer S (PEQLAB Biotechnologie GmbH, Erlangen, Germany), 1 μl of 25 mM MgCl2 (PEQLAB Biotechnologie GmbH, Erlangen, Germany), 0.625 μl of a 10 mM dNTP mix (PEQLAB Biotechnologie GmbH, Erlangen, Germany), 0.75 μl of 3 mg ml-1 bovine serum albumin (Sigma-Aldrich Chemie Gmbh; Munich, Germany), 0.25 μl of 40 μM universal forward primer ITSF (5’- GTCGTAACAAGGTAGCCGTA-3’) (Biomers.net, Ulm, Germany), labeled with 6- carboxyfluorescein (FAM), 0.25 μl of 40 μM ITSReub reverse primer (5’- GCCAAGGCATCCACC-3’) (Biomers.net, Ulm, Germany), 0.25 μl of 5 units μl-1 Taq polymerase (PEQLAB Biotechnologie GmbH, Erlangen, Germany) and approximately 10 ng environmental DNA. ..
Article Title: Diazotroph Diversity in the Sea Ice, Melt Ponds, and Surface Waters of the Eurasian Basin of the Central Arctic Ocean
Article Snippet: .. Each ARISA PCR contained 2.5 μl of 10x reaction buffer S (PEQLAB Biotechnologie GmbH, Erlangen, Germany), 1 μl of 25 mM MgCl 2 (PEQLAB Biotechnologie GmbH, Erlangen, Germany), 0.625 μl of a 10 mM dNTP mix (PEQLAB Biotechnologie GmbH, Erlangen, Germany), 0.75 μl of 3 mg ml -1 bovine serum albumin (Sigma-Aldrich Chemie Gmbh; Munich, Germany), 0.25 μl of 40 μM universal forward primer ITSF (5′-GTCGTAACAAGGTAGCCGTA-3′) (Biomers.net, Ulm, Germany), labeled with 6-carboxyfluorescein (FAM), 0.25 μl of 40 μM ITSReub reverse primer (5′-GCCAAGGCATCCACC-3′) (Biomers.net, Ulm, Germany), 0.25 μl of 5 units μl -1 Taq polymerase (PEQLAB Biotechnologie GmbH, Erlangen, Germany) and approximately 10 ng environmental DNA as determined by spectrophotometry (Infinite ® M200 NanoQuant, Tecan Group Ltd., Switzerland). ..
Labeling:Article Title: Effects of Ice-Algal Aggregate Export on the Connectivity of Bacterial Communities in the Central Arctic Ocean
Article Snippet: .. The PCR mix for the ITS region contained 2.5 μl of 10x reaction buffer S (PEQLAB Biotechnologie GmbH, Erlangen, Germany), 1 μl of 25 mM MgCl2 (PEQLAB Biotechnologie GmbH, Erlangen, Germany), 0.625 μl of a 10 mM dNTP mix (PEQLAB Biotechnologie GmbH, Erlangen, Germany), 0.75 μl of 3 mg ml-1 bovine serum albumin (Sigma-Aldrich Chemie Gmbh; Munich, Germany), 0.25 μl of 40 μM universal forward primer ITSF (5’- GTCGTAACAAGGTAGCCGTA-3’) (Biomers.net, Ulm, Germany), labeled with 6- carboxyfluorescein (FAM), 0.25 μl of 40 μM ITSReub reverse primer (5’- GCCAAGGCATCCACC-3’) (Biomers.net, Ulm, Germany), 0.25 μl of 5 units μl-1 Taq polymerase (PEQLAB Biotechnologie GmbH, Erlangen, Germany) and approximately 10 ng environmental DNA. ..
Article Title: Diazotroph Diversity in the Sea Ice, Melt Ponds, and Surface Waters of the Eurasian Basin of the Central Arctic Ocean
Article Snippet: .. Each ARISA PCR contained 2.5 μl of 10x reaction buffer S (PEQLAB Biotechnologie GmbH, Erlangen, Germany), 1 μl of 25 mM MgCl 2 (PEQLAB Biotechnologie GmbH, Erlangen, Germany), 0.625 μl of a 10 mM dNTP mix (PEQLAB Biotechnologie GmbH, Erlangen, Germany), 0.75 μl of 3 mg ml -1 bovine serum albumin (Sigma-Aldrich Chemie Gmbh; Munich, Germany), 0.25 μl of 40 μM universal forward primer ITSF (5′-GTCGTAACAAGGTAGCCGTA-3′) (Biomers.net, Ulm, Germany), labeled with 6-carboxyfluorescein (FAM), 0.25 μl of 40 μM ITSReub reverse primer (5′-GCCAAGGCATCCACC-3′) (Biomers.net, Ulm, Germany), 0.25 μl of 5 units μl -1 Taq polymerase (PEQLAB Biotechnologie GmbH, Erlangen, Germany) and approximately 10 ng environmental DNA as determined by spectrophotometry (Infinite ® M200 NanoQuant, Tecan Group Ltd., Switzerland). ..
Spectrophotometry:Article Title: Diazotroph Diversity in the Sea Ice, Melt Ponds, and Surface Waters of the Eurasian Basin of the Central Arctic Ocean
Article Snippet: .. Each ARISA PCR contained 2.5 μl of 10x reaction buffer S (PEQLAB Biotechnologie GmbH, Erlangen, Germany), 1 μl of 25 mM MgCl 2 (PEQLAB Biotechnologie GmbH, Erlangen, Germany), 0.625 μl of a 10 mM dNTP mix (PEQLAB Biotechnologie GmbH, Erlangen, Germany), 0.75 μl of 3 mg ml -1 bovine serum albumin (Sigma-Aldrich Chemie Gmbh; Munich, Germany), 0.25 μl of 40 μM universal forward primer ITSF (5′-GTCGTAACAAGGTAGCCGTA-3′) (Biomers.net, Ulm, Germany), labeled with 6-carboxyfluorescein (FAM), 0.25 μl of 40 μM ITSReub reverse primer (5′-GCCAAGGCATCCACC-3′) (Biomers.net, Ulm, Germany), 0.25 μl of 5 units μl -1 Taq polymerase (PEQLAB Biotechnologie GmbH, Erlangen, Germany) and approximately 10 ng environmental DNA as determined by spectrophotometry (Infinite ® M200 NanoQuant, Tecan Group Ltd., Switzerland). ..
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